RT Journal Article
SR Electronic
T1 <em>Nardostachys jatamansi</em> Root Extract Attenuates Tumor Progression in Hepatocellular Carcinoma <em>via</em> Inhibition of ERK/STAT3 Pathways
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1883
OP 1893
DO 10.21873/anticanres.14954
VO 41
IS 4
A1 LEE, GIL WON
A1 HUR, WONHEE
A1 KIM, JUNG-HEE
A1 PARK, DONG JUN
A1 KIM, SUNG MIM
A1 KANG, BYUNG-YOON
A1 SUNG, PIL SOO
A1 YOON, SEUNG KEW
YR 2021
UL http://ar.iiarjournals.org/content/41/4/1883.abstract
AB Background/Aim: Hepatocellular carcinoma (HCC) is a highly prevalent disease and treatment is limited. Therefore, development of new therapeutic agents is urgent. The aim of this study was to investigate the in vitro and in vivo anti-cancer effects of Nardostachys jatamansi root extract (NJRE) against HCC and underlying mechanisms involved in such effects. Materials and Methods: Effects of NJRE on viability of HCC cell lines were determined by MTT analysis and annexin/PI apoptosis assays. Expression levels of proteins in MAPK and STAT3 pathways and caspase-3 and PARP after treatment with NJRE in HCC cell lines were determined by western blotting. In a syngeneic model using mouse HCC cells Hepa1-6, inhibition of tumor formation after oral administration of NJRE was determined and expression levels of phospho-ERK and phospho-STAT3 in liver tissues were analyzed by immunohistochemical staining. Results: NJRE reduced the activation of STAT3 by inhibiting the expression of ERK and finally attenuated the proliferation of HCC. Conclusion: NJRE has anti-cancer effects against HCC. It has potential to be used in the treatment of human HCC.