PT  - JOURNAL ARTICLE
AU  - JIAN NI
AU  - KOICHI TAKAYAMA
AU  - NAOKO INOSHIMA
AU  - JUNJI UCHINO
AU  - AKIKO HARADA
AU  - TAKAHIRO MINAMI
AU  - TAISHI HARADA
AU  - CAICUN ZHOU
AU  - YOICHI NAKANISHI
TI  - Gene Transfer of Inhibitor KappaB in Human Lung Cancer Cell Line NCI-H460 Inhibits Tumorigenesis and Angiogenesis <em>In Vivo</em>
DP  - 2005 Jan 01
TA  - Anticancer Research
PG  - 69--77
VI  - 25
IP  - 1A
4099  - http://ar.iiarjournals.org/content/25/1A/69.short
4100  - http://ar.iiarjournals.org/content/25/1A/69.full
SO  - Anticancer Res2005 Jan 01; 25
AB  - Background: Nuclear factor kappaB (NFÎ B) is an inducible and ubiquitously expressed transcription factor which is involved in cell survival, differentiation and growth and, thus, has also been implicated in tumor formation and development. Research on the effect of NFÎ B in inhibiting cancer cell growth, however, remains controversial. Materials and Methods: We investigated the effects of overexpressed IÎ Bα on the proliferation of the human lung cancer cell line H460 in vitro and in vivo using IÎ Bα-expressing adenovirus. Results: The results suggested that the infection of AdIÎ Bα blocked NFÎ B activity in H460 cells and significantly inhibited cell proliferation by inducing apoptosis. An in vivo study showed the tumor incidence to be significantly lower in mice implanted with H460 cells infected with AdIÎ Bα. For established H460 tumor, the intratumoral injection of AdIÎ Bα also inhibited the tumor growth due to both a blockade of the NFÎ B activity and an inhibition of the VEGF expression. Conclusion: Adenovirus-mediated IÎ Bα gene transfer is a promising cancer treatment strategy.