TY - JOUR T1 - Sorafenib Induces Apoptosis and Inhibits NF-κB-mediated Anti-apoptotic and Metastatic Potential in Osteosarcoma Cells JF - Anticancer Research JO - Anticancer Res SP - 1251 LP - 1259 DO - 10.21873/anticanres.14882 VL - 41 IS - 3 AU - CHING-HSUAN WU AU - KUNG-HUNG LIN AU - BO-SIANG FU AU - FEI-TING HSU AU - JAI-JEN TSAI AU - MAO-CHI WENG AU - PO-JUNG PAN Y1 - 2021/03/01 UR - http://ar.iiarjournals.org/content/41/3/1251.abstract N2 - Background/Aim: Sorafenib, an oral multi-kinase inhibitor, has been shown to improve the outcome of patients with osteosarcoma (OS). However, the anti-OS effect and mechanism of sorafenib has not yet been fully understood. The main purpose of this study was to investigate the effect of sorafenib on apoptotic signaling and Nuclear Factor-κB (NF-κB)-mediated anti-apoptotic and metastatic potential in OS in vitro. Materials and Methods: The effect of sorafenib on apoptotic signaling transduction, anti-apoptotic, and metastatic potential of OS U-2 cells was verified with flow cytometry, trans-well invasion/migration, and western blotting assay. Results: Sorafenib induced the extrinsic and intrinsic apoptotic pathways. In addition, sorafenib reduced the invasion and migration ability of OS cells, induced NF-κB activation, and the expression of anti-apoptotic proteins and metastasis-associated proteins encoded by NF-κB target genes. Conclusion: Sorafenib led to stimulation of extrinsic/intrinsic apoptotic pathways and NF-κB inactivation in U-2 OS cells. ER -