RT Journal Article
SR Electronic
T1 Reversion from Methionine Addiction to Methionine Independence Results in Loss of Tumorigenic Potential of Highly-malignant Lung-cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 641
OP 643
DO 10.21873/anticanres.14815
VO 41
IS 2
A1 JUN YAMAMOTO
A1 YUSUKE AOKI
A1 QINGHONG HAN
A1 NORIHIKO SUGISAWA
A1 YU SUN
A1 KAZUYUKI HAMADA
A1 HIROTO NISHINO
A1 SACHIKO INUBUSHI
A1 KENTARO MIYAKE
A1 RYUSEI MATSUYAMA
A1 MICHAEL BOUVET
A1 ITARU ENDO
A1 ROBERT M. HOFFMAN
YR 2021
UL http://ar.iiarjournals.org/content/41/2/641.abstract
AB Background/Aim: Methionine addiction, a fundamental and general hallmark of cancer, is due to the excess use of methionine for transmethylation, and is described as the Hoffman-effect. Methionine-addicted cancer cells can revert at low frequency to methionine independence when selected under methionine-restriction. We report here that highly-malignant methionine-addicted H460 human lung-cancer cells, when selected for methionine independence, have greatly-reduced tumorigenic potential. Materials and Methods: Methionine-addicted H460 parental cancer cells and methionine-independent revertant H460-R1 cells were injected in nude mice subcutaneously. Results: When the parental H460 methionine-addicted cells were injected in nude mice at 2.5×105, 1×105 and 5×104, the cells could form tumors. In contrast, the H460-R1 methionine-independent revertant cells could not form tumors when the above-listed cell numbers were injected in nude mice. Conclusion: There is a tight linkage between methionine addiction and malignancy.