RT Journal Article
SR Electronic
T1 A Novel Orthotopic Mouse Model of Lung Metastasis Using Fluorescent Patient-derived Osteosarcoma Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 635
OP 640
DO 10.21873/anticanres.14814
VO 41
IS 2
A1 OSHIRO, HIROMICHI
A1 TOME, YASUNORI
A1 MIYAKE, KENTARO
A1 HIGUCHI, TAKASHI
A1 SUGISAWA, NORIHIKO
A1 PARK, JUN HO
A1 ZHANG, ZHIYING
A1 RAZMJOOEI, SAHAR
A1 KANAYA, FUMINORI
A1 NISHIDA, KOTARO
A1 HOFFMAN, ROBERT M.
YR 2021
UL http://ar.iiarjournals.org/content/41/2/635.abstract
AB Background: A mouse model of metastatic osteosarcoma is imperative to identify effective agents for metastatic osteosarcoma, which is a recalcitrant disease. In the present study, we established osteosarcoma patient-derived cells (OS-PDCs) and transfected them with green fluorescent protein (GFP). Materials and Methods: The OS-PDCs were transfected with GFP-lentivirus. GFP-expressing OS-PDCs (2.0×105) were then injected into the tibia of nude mice to establish the patient-derived orthotopic cell (PDOC) model (n=3). Six weeks after injection, the primary tumor and each organ were resected and imaged. Results: Primary orthotopic tumors were established in two out of three mice. The GFP-expressing OS-PDCs in the PDOC model were visualized. Multiple GFP-expressing lung metastases were detected in one of the two mice with primary tumor. Conclusion: The present study proves the concept that a GFP-expressing PDOC model can mimic clinical lung-metastatic osteosarcoma. This model can serve as a paradigm to screen for effective drugs for osteosarcoma lung metastasis.