PT  - JOURNAL ARTICLE
AU  - HIROMICHI OSHIRO
AU  - YASUNORI TOME
AU  - KENTARO MIYAKE
AU  - TAKASHI HIGUCHI
AU  - NORIHIKO SUGISAWA
AU  - JUN HO PARK
AU  - ZHIYING ZHANG
AU  - SAHAR RAZMJOOEI
AU  - FUMINORI KANAYA
AU  - KOTARO NISHIDA
AU  - ROBERT M. HOFFMAN
TI  - A Novel Orthotopic Mouse Model of Lung Metastasis Using Fluorescent Patient-derived Osteosarcoma Cells
AID  - 10.21873/anticanres.14814
DP  - 2021 Feb 01
TA  - Anticancer Research
PG  - 635--640
VI  - 41
IP  - 2
4099  - http://ar.iiarjournals.org/content/41/2/635.short
4100  - http://ar.iiarjournals.org/content/41/2/635.full
SO  - Anticancer Res2021 Feb 01; 41
AB  - Background: A mouse model of metastatic osteosarcoma is imperative to identify effective agents for metastatic osteosarcoma, which is a recalcitrant disease. In the present study, we established osteosarcoma patient-derived cells (OS-PDCs) and transfected them with green fluorescent protein (GFP). Materials and Methods: The OS-PDCs were transfected with GFP-lentivirus. GFP-expressing OS-PDCs (2.0×105) were then injected into the tibia of nude mice to establish the patient-derived orthotopic cell (PDOC) model (n=3). Six weeks after injection, the primary tumor and each organ were resected and imaged. Results: Primary orthotopic tumors were established in two out of three mice. The GFP-expressing OS-PDCs in the PDOC model were visualized. Multiple GFP-expressing lung metastases were detected in one of the two mice with primary tumor. Conclusion: The present study proves the concept that a GFP-expressing PDOC model can mimic clinical lung-metastatic osteosarcoma. This model can serve as a paradigm to screen for effective drugs for osteosarcoma lung metastasis.