TY - JOUR T1 - The Blood Microenvironment Influences the Molecular Phenotypes of Circulating Tumor Cells in Head and Neck Squamous Cell Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 885 LP - 893 DO - 10.21873/anticanres.14841 VL - 41 IS - 2 AU - HIROE TADA AU - HIDEYUKI TAKAHASHI AU - SHOTA IDA AU - IKKO MITO AU - TOSHIYUKI MATSUYAMA AU - KAZUAKI CHIKAMATSU Y1 - 2021/02/01 UR - http://ar.iiarjournals.org/content/41/2/885.abstract N2 - Background/Aim: Circulating tumor cells (CTCs) may be affected by the environment encountered during blood circulation. We aimed to explore the association between the molecular phenotype of CTCs and systemic inflammatory markers. Patients and Methods: CTCs isolated from patients with recurrent/metastatic head and neck squamous cell carcinoma by CD45-negative selection were analyzed for the expression of multiple genes. The correlations between gene expression levels in CTCs and systemic inflammation markers were examined. Results: Thirty-five (83.3%) of the 42 patients were positive for CTCs. No significant differences in systemic inflammatory markers were observed between CTC-positive and CTC-negative patients. Notably, VIM or ZEB2 expression was strongly correlated with that of CD44 or ALDH1. PIK3CA, CD44, ALDH1A1, and PDCD1LG2 expression in CTCs was correlated with lymphocyte- and/or albumin-related systemic inflammatory markers. Conclusion: CTCs acquire a survival advantage through phenotypic alterations in the hostile blood environment, and evade circulatory immune surveillance. ER -