PT - JOURNAL ARTICLE AU - VAN ETTEN, BOUDEWIJN AU - VAN TIEL, SANDRA T. AU - AMBAGTSHEER, GISELA AU - EGGERMONT, ALEXANDER M.M. AU - HAGEN, TIMO L.M. TEN TI - Isolated Limb Perfusion Based Anti-p21<em>ras</em> Gene Therapy in a Rat Rhabdomyosarcoma DP - 2004 Jul 01 TA - Anticancer Research PG - 2295--2302 VI - 24 IP - 4 4099 - http://ar.iiarjournals.org/content/24/4/2295.short 4100 - http://ar.iiarjournals.org/content/24/4/2295.full SO - Anticancer Res2004 Jul 01; 24 AB - Background: Inhibition of ras oncogene is a promising new strategy. Gene therapy against ras proved successful in human and murine tumour cell lines. Previously we demonstrated effective targeted transfection of tumour in a rat model by using an isolated limb perfusion (ILP) for the delivery of adenoviral vectors. Materials and Methods: This study explores the anti-tumour activity of an adenoviral construct encoding an intracellular single-chain antibody (scFv) against p21ras (Y28). In order to determine the influence of the ras status on the efficacy of the scFv, we used a wild-type rat rhabdomyosarcoma and its ras-oncogene transfectant, for in vitro studies. In vivo we used the ILP delivery method to study anti-tumour activity on established limb tumours. Results: In vitro studies demonstrated an inhibition of growth caused by the Y28 construct. No significant difference between transfected and wild-type cell lines could be demonstrated. Upon ILP, homogeneous transduction was observed in 5% of tumour cells. Perfusion with the Y28 construct, however, did not result in any additional anti-tumour activity compared to controls. Conclusion: Despite in vitro activity and in vivo transfection, no significant tumour response could be detected using anti-p21ras gene therapy in this ILP-tumour model. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved