TY - JOUR T1 - Rapid Flow Cytometry of Gastrointestinal Stromal Tumours Closely Matches the Modified Fletcher Classification JF - Anticancer Research JO - Anticancer Res SP - 131 LP - 136 DO - 10.21873/anticanres.14758 VL - 41 IS - 1 AU - KIYOAKI TANIGUCHI AU - AKANE SUZUKI AU - AKIKO SERIZAWA AU - SHO KOTAKE AU - SHUNICHI ITO AU - KAZUOMI SUZUKI AU - TAKUJI YAMADA AU - TAKEHARU NOGUCHI AU - KUNIHIKO AMANO AU - MASAHO OTA AU - YOSHIHIRO MURAGAKI AU - MASAKAZU YAMAMOTO Y1 - 2021/01/01 UR - http://ar.iiarjournals.org/content/41/1/131.abstract N2 - Aim: We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use. Materials and Methods: Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification. Results: We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk. Conclusion: Rapid flow cytometry parameters can classify risk without the need for histological analysis. ER -