%0 Journal Article %A KIYOAKI TANIGUCHI %A AKANE SUZUKI %A AKIKO SERIZAWA %A SHO KOTAKE %A SHUNICHI ITO %A KAZUOMI SUZUKI %A TAKUJI YAMADA %A TAKEHARU NOGUCHI %A KUNIHIKO AMANO %A MASAHO OTA %A YOSHIHIRO MURAGAKI %A MASAKAZU YAMAMOTO %T Rapid Flow Cytometry of Gastrointestinal Stromal Tumours Closely Matches the Modified Fletcher Classification %D 2021 %R 10.21873/anticanres.14758 %J Anticancer Research %P 131-136 %V 41 %N 1 %X Aim: We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use. Materials and Methods: Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification. Results: We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk. Conclusion: Rapid flow cytometry parameters can classify risk without the need for histological analysis. %U https://ar.iiarjournals.org/content/anticanres/41/1/131.full.pdf