RT Journal Article
SR Electronic
T1 MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 137
OP 149
DO 10.21873/anticanres.14759
VO 41
IS 1
A1 ALEXANDRA KISS
A1 VIKTÓRIA BAKSA
A1 MIKLÓS BEGE
A1 LÁSZLÓ TÁLAS
A1 ANIKÓ BORBÁS
A1 ILONA BERECZKI
A1 GÁSPÁR BÁNFALVI
A1 GÁBOR SZEMÁN-NAGY
YR 2021
UL http://ar.iiarjournals.org/content/41/1/137.abstract
AB Background/Aim: Conventional viability tests, help to screen the cellular effects of candidate molecules, but the endpoint of these measurements lacks sufficient information regarding the molecular aspects. A non-invasive, easy-to-setup live-cell microscopic method served to in-depth analysis of mechanisms of potential anticancer drugs. Materials and Methods: The proposed method combining the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test with time-lapse scanning microscopy (TLS), provided additional data related to the cell-cycle and the dynamic properties of cell morphology. Apoptotic and necrotic events became detectable with these methods. Results: Quantification of the results was assisted by image analysis of the acquired image sequences. After demonstrating the potential of the TLS method, a series of experiments compared the in vitro effect of a known and a newly synthesized nucleoside analogue. Conclusion: The proposed approach provided a more in-depth insight into the cellular processes that can be affected by known chemotherapeutic agents including nucleoside analogues rather than applying repeated individual treatments.