PT - JOURNAL ARTICLE AU - ALEXANDRA KISS AU - VIKTÓRIA BAKSA AU - MIKLÓS BEGE AU - LÁSZLÓ TÁLAS AU - ANIKÓ BORBÁS AU - ILONA BERECZKI AU - GÁSPÁR BÁNFALVI AU - GÁBOR SZEMÁN-NAGY TI - MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues AID - 10.21873/anticanres.14759 DP - 2021 Jan 01 TA - Anticancer Research PG - 137--149 VI - 41 IP - 1 4099 - http://ar.iiarjournals.org/content/41/1/137.short 4100 - http://ar.iiarjournals.org/content/41/1/137.full SO - Anticancer Res2021 Jan 01; 41 AB - Background/Aim: Conventional viability tests, help to screen the cellular effects of candidate molecules, but the endpoint of these measurements lacks sufficient information regarding the molecular aspects. A non-invasive, easy-to-setup live-cell microscopic method served to in-depth analysis of mechanisms of potential anticancer drugs. Materials and Methods: The proposed method combining the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test with time-lapse scanning microscopy (TLS), provided additional data related to the cell-cycle and the dynamic properties of cell morphology. Apoptotic and necrotic events became detectable with these methods. Results: Quantification of the results was assisted by image analysis of the acquired image sequences. After demonstrating the potential of the TLS method, a series of experiments compared the in vitro effect of a known and a newly synthesized nucleoside analogue. Conclusion: The proposed approach provided a more in-depth insight into the cellular processes that can be affected by known chemotherapeutic agents including nucleoside analogues rather than applying repeated individual treatments.