TY - JOUR T1 - MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues JF - Anticancer Research JO - Anticancer Res SP - 137 LP - 149 DO - 10.21873/anticanres.14759 VL - 41 IS - 1 AU - ALEXANDRA KISS AU - VIKTÓRIA BAKSA AU - MIKLÓS BEGE AU - LÁSZLÓ TÁLAS AU - ANIKÓ BORBÁS AU - ILONA BERECZKI AU - GÁSPÁR BÁNFALVI AU - GÁBOR SZEMÁN-NAGY Y1 - 2021/01/01 UR - http://ar.iiarjournals.org/content/41/1/137.abstract N2 - Background/Aim: Conventional viability tests, help to screen the cellular effects of candidate molecules, but the endpoint of these measurements lacks sufficient information regarding the molecular aspects. A non-invasive, easy-to-setup live-cell microscopic method served to in-depth analysis of mechanisms of potential anticancer drugs. Materials and Methods: The proposed method combining the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test with time-lapse scanning microscopy (TLS), provided additional data related to the cell-cycle and the dynamic properties of cell morphology. Apoptotic and necrotic events became detectable with these methods. Results: Quantification of the results was assisted by image analysis of the acquired image sequences. After demonstrating the potential of the TLS method, a series of experiments compared the in vitro effect of a known and a newly synthesized nucleoside analogue. Conclusion: The proposed approach provided a more in-depth insight into the cellular processes that can be affected by known chemotherapeutic agents including nucleoside analogues rather than applying repeated individual treatments. ER -