TY - JOUR T1 - Color Coding Cancer Cells with Fluorescent Proteins to Visualize <em>In Vivo</em> Cellular Interaction in Metastatic Colonies JF - Anticancer Research JO - Anticancer Res SP - 4067 LP - 4072 VL - 24 IS - 6 AU - NORIO YAMAMOTO AU - MENG YANG AU - PING JIANG AU - MINGXU XU AU - KENSUKE YAMAUCHI AU - HIROYUKI TSUCHIYA AU - KATSURO TOMITA AU - A.R. MOOSSA AU - ROBERT M. HOFFMAN Y1 - 2004/11/01 UR - http://ar.iiarjournals.org/content/24/6/4067.abstract N2 - We report here in vivo visualization of cancer cell interaction using stable sublines of green fluorescent protein (GFP)- and red fluorescent protein (RFP)-expressing HT-1080 human fibrosarcoma cells. These color-coded fibrosarcoma cell lines show similar cell proliferation and lung metastasis potential. The color-coded cells were mixed at a ratio of 1:1 and were injected into the tail vein of severe combined immunodeficient (SCID) mice. The resulting experimental lung metastases were simultaneously imaged and their ratio was determined by color-pixel analysis. Fluorescence color coding of cancer cells enables visualization of the interaction of cancer cells and can be used to distinguish cancer cells of any genotype or phenotype. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -