PT - JOURNAL ARTICLE AU - AFSANEH SORURI AU - AFSHIN FAYYAZI AU - STEFANIE GANGL AU - CHRISTIAN GRIESINGER AU - CHRISTIAN A. ALBRECHT AU - THILO SCHLOTT TI - <em>In Vitro</em> Generation of Cytolytic T Cells Against Human Melanoma Cells Overexpressing HDM2 DP - 2004 Jul 01 TA - Anticancer Research PG - 2141--2152 VI - 24 IP - 4 4099 - http://ar.iiarjournals.org/content/24/4/2141.short 4100 - http://ar.iiarjournals.org/content/24/4/2141.full SO - Anticancer Res2004 Jul 01; 24 AB - Background: Previous experiments have shown that tumour-associated antigens can be exploited for a successful anti-tumour immunisation. Previous reports demonstrated that oncoprotein MDM2 (HDM2) contains two highly conserved MHC class I binding motifs, MDM2100 and MDM2441, and that dendritic cells (DC) presenting MDM2100 stimulate an effective CTL reaction against melanoma cells. Materials and Methods: In this study, we investigated the CTL-inducing capacity of autologous human dendritic cells pulsed with fragment HDM2441. Results: In vitro HDM2441-primed T lymphocytes revealed a strong proliferation activity, released Th-1-associated cytokines, and possessed an effective anti-tumour activity causing apoptosis in HDM2441-overexpressing melanoma cells. Cytotoxic assay demonstrated that, in parallel to melanoma cells, up to 65% of primed T cells also underwent apoptosis. Conclusion: These data suggest that HDM2441 may be exploited for broad-spectrum DC-based trials against metastatic melanomas overexpressing HDM2, and point out that the efficacy of such immunotherapeutical approaches may be limited via T cell apoptosis. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved