@article {SORURI2141, author = {AFSANEH SORURI and AFSHIN FAYYAZI and STEFANIE GANGL and CHRISTIAN GRIESINGER and CHRISTIAN A. ALBRECHT and THILO SCHLOTT}, title = {In Vitro Generation of Cytolytic T Cells Against Human Melanoma Cells Overexpressing HDM2}, volume = {24}, number = {4}, pages = {2141--2152}, year = {2004}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Previous experiments have shown that tumour-associated antigens can be exploited for a successful anti-tumour immunisation. Previous reports demonstrated that oncoprotein MDM2 (HDM2) contains two highly conserved MHC class I binding motifs, MDM2100 and MDM2441, and that dendritic cells (DC) presenting MDM2100 stimulate an effective CTL reaction against melanoma cells. Materials and Methods: In this study, we investigated the CTL-inducing capacity of autologous human dendritic cells pulsed with fragment HDM2441. Results: In vitro HDM2441-primed T lymphocytes revealed a strong proliferation activity, released Th-1-associated cytokines, and possessed an effective anti-tumour activity causing apoptosis in HDM2441-overexpressing melanoma cells. Cytotoxic assay demonstrated that, in parallel to melanoma cells, up to 65\% of primed T cells also underwent apoptosis. Conclusion: These data suggest that HDM2441 may be exploited for broad-spectrum DC-based trials against metastatic melanomas overexpressing HDM2, and point out that the efficacy of such immunotherapeutical approaches may be limited via T cell apoptosis. Copyright{\textcopyright} 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/24/4/2141}, eprint = {https://ar.iiarjournals.org/content/24/4/2141.full.pdf}, journal = {Anticancer Research} }