RT Journal Article
SR Electronic
T1 Effect of Estrogen, Tamoxifen and Epidermal Growth Factor on the Transcriptional Regulation of Vascular Endothelial Growth Factor in Breast Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3961
OP 3964
VO 24
IS 6
A1 JEONG EON LEE
A1 KI-WOOK CHUNG
A1 WONSHIK HAN
A1 SUNG-WON KIM
A1 SEOK WON KIM
A1 HYUK JAI SHIN
A1 JI YEOUN BAE
A1 DONG-YOUNG NOH
YR 2004
UL http://ar.iiarjournals.org/content/24/6/3961.abstract
AB Background: VEGF (Vascular Endothelial Growth Factor) is a key factor of angiogenesis and high tissue VEGF levels are related to a poor prognosis in breast cancer. Materials and Methods: By semi-quantitative RT-PCR, we determined the relative expressions of VEGF mRNA in MCF-7 (both ER-α+ and ER-β+ (mainly ER-α+), PR+, bcl-2+, EGFR-) and MB-MDA-231 (only ER-β+, PR-, EGFR-) breast cancer cells which were treated with estrogen, tamoxifen and EGF (Epidermal Growth Factor). Results: In MCF-7 cell lines, estrogen induced the expression of VEGF mRNA while tamoxifen reduced its expression. Estrogen and tamoxifen did not confer any significant effect on MB-MDA-231 cells and EGF showed no significant effect on MCF-7 or MB-MDA-231. Conclusion: Reduced VEGF mRNA expression of MCF-7 cells treated with tamoxifen may be related to the antagonistic effect of tamoxifen on ER-positive breast cancer, and this antagonistic effect may be related to ER-α. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved