RT Journal Article SR Electronic T1 Cytology-based Detection of Circulating Tumour Cells in Human Pancreatic Cancer Xenograft Models With KRAS Mutation JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6781 OP 6789 DO 10.21873/anticanres.14701 VO 40 IS 12 A1 ITO, YUKAKO A1 INOUE, ERIKO A1 MATSUI, YUKI A1 KOBUCHI, SHINJI A1 MOYAMA, CHIAMI A1 AMAGASE, KIKUKO A1 YOSHIMURA, MAYUMI A1 IKEHARA, YUZURU A1 NAKATA, SUSUMU A1 NAKANISHI, HAYAO YR 2020 UL http://ar.iiarjournals.org/content/40/12/6781.abstract AB Background/Aim: To examine the dynamics of circulating tumour cells (CTCs) in pancreatic cancer (PC), new mouse CTC models from human PC xenografts were developed. Materials and Methods: Orthotopic (pancreas) and heterotopic (subcutaneous) transplantation models using GFP-tagged SUIT-2 PC cells were prepared. Using a cytology-based CTC detection platform, CTCs and metastasis were compared. Results: The two types of orthotopic models, including the surgical transplantation model and the intraperitoneal injection model, showed a similar pattern of initial pancreatic tumour formation and subsequent development of peritoneal and hematogenous lung metastases. In the heterotopic model, only hematogenous lung metastasis was observed, and the number of CTCs and lung metastases was higher than that of the orthotopic model. Furthermore, KRAS mutation (G12D) was detected in CTCs. Conclusion: These orthotopic and heterotopic models clearly differ in terms of the pattern of metastasis and CTCs and therefore, would be useful PC models to investigate the effect of drug-therapy on CTCs and the role of KRAS mutation.