PT  - JOURNAL ARTICLE
AU  - JI-HYE YOON
AU  - SUNG-GOOK CHO
TI  - ADGRF4 Regulates Non-small Cell Lung Cancer Cell Invasiveness
AID  - 10.21873/anticanres.14705
DP  - 2020 Dec 01
TA  - Anticancer Research
PG  - 6835--6844
VI  - 40
IP  - 12
4099  - http://ar.iiarjournals.org/content/40/12/6835.short
4100  - http://ar.iiarjournals.org/content/40/12/6835.full
SO  - Anticancer Res2020 Dec 01; 40
AB  - Background/Aim: Adhesion G protein-coupled receptors (aGPCRs) have a crucial role in cancer. However, the role of ADGRF4, one of aGPCRs, in cancer has yet to be revealed. Therefore, we investigated its role in lung cancer, a leading cause of cancer-related deaths worldwide. Materials and Methods: ADGRF4 gene expression pattern in lung cancer were analyzed by in silico analyses. RNA sequencing was conducted to investigate gene expression pattern altered by ADGRF4 knockdown. Lung cancer cell lines were subjected to cell migration and invasion assays. Results: In silico analysis data indicated a major role of ADGRF4 in lung cancer. RNA sequencing data showed that ADGRF4 gene silencing in lung cancer cells altered global expression pattern. ADGRF4 gene silencing reduced lung cancer cell invasiveness. Furthermore, PPP2C gene expression was most significantly down-regulated by ADGRF4 gene silencing. PPP2C overexpression rescued cell invasiveness inhibited by ADGRF4 gene silencing, and PPP2C gene silencing blocked lung cancer cell invasiveness. Conclusion: ADGRF4 regulates lung cancer cell invasiveness via PPP2C.