RT Journal Article SR Electronic T1 Novel Prognostic Implications of Methylated RNA and Demethylases in Resected HCC and Background Liver Tissue JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6665 OP 6676 DO 10.21873/anticanres.14690 VO 40 IS 12 A1 NOBUHIKO NAKAGAWA A1 KATSUHITO TANAKA A1 FUMINORI SONOHARA A1 RAJU KANDIMALLA A1 YUKI SUNAGAWA A1 YOSHIKUNI INOKAWA A1 HIDEKI TAKAMI A1 MASAMICHI HAYASHI A1 SUGURU YAMADA A1 MITSURO KANDA A1 CHIE TANAKA A1 GORO NAKAYAMA A1 MASAHIKO KOIKE A1 YASUHIRO KODERA YR 2020 UL http://ar.iiarjournals.org/content/40/12/6665.abstract AB Background/Aim: N6-Methyladenosine (m6A), the most abundant internal modification of RNA, plays a critical role in cancer development. However, the clinical implications of m6A in hepatocellular carcinoma (HCC) remain unclear. Materials and Methods: We analyzed 177 HCC and paired noncancerous liver tissues from patients who underwent hepatectomy according to global m6A quantification and expression of m6A demethylases fat mass and obesity-associated protein (FTO) and alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 (ALKBH5). Results: The global m6A quantification revealed no significant difference between HCC and non-cancerous tissue. The expression of m6A demethylases FTO and ALKBH5, was significantly lower in HCC than in non-cancerous tissues (both p<0.001). Furthermore, low ALKBH5 expression in non-cancerous tissues was significantly correlated with worse recurrence-free survival (median of 16.3 vs. 38.9 months, p=0.001). Conclusion: m6A in HCC and its demethylase in surrounding non-cancerous liver tissues might be involved in inherent mechanisms for HCC development and affect malignant potential after HCC resection.