PT - JOURNAL ARTICLE AU - JUN YAMAMOTO AU - NORIHIKO SUGISAWA AU - KAZUYUKI HAMADA AU - HIROTO NISHINO AU - KENTARO MIYAKE AU - RYUSEI MATSUYAMA AU - SACHIKO INUBUSHI AU - HIROKAZU TANINO AU - MICHAEL BOUVET AU - ITARU ENDO AU - ROBERT M. HOFFMAN TI - A Universal Gelfoam 3-D Histoculture Method to Establish Patient-derived Cancer Cells (3D-PDCC) Without Fibroblasts from Patient-derived Xenografts AID - 10.21873/anticanres.14699 DP - 2020 Dec 01 TA - Anticancer Research PG - 6765--6768 VI - 40 IP - 12 4099 - http://ar.iiarjournals.org/content/40/12/6765.short 4100 - http://ar.iiarjournals.org/content/40/12/6765.full SO - Anticancer Res2020 Dec 01; 40 AB - Background/Aim: The direct placement of patient tumors in 2-D culture on plastic or glass surfaces has inhibited the establishment of patient-derived cancer cells (PDCCs). The aim of the present study was to develop universal and efficient methods to prepare PDCCs. Materials and Methods: Fragments of patient-derived xenograft (PDX) tumors established form colon cancer liver metastasis (1 mm3) were placed on Gelfoam and cultured in DMEM. Results: PDX tumor fragments were cultured on Gelfoam. Cancer cells migrated from the explant and formed distinct 3-D structures in the Gelfoam. Each of the three PDCCs showed a distinct morphology. The cultures were essentially all cancer cells without fibroblasts, the opposite of what usually occurs in 2-D culture on plastic or glass. Gelfoam cultures could be readily passaged from one Gelfoam cube to anothers suggesting indefinite culture potential. Conclusion: A potentially universal method to establish PDCC using PDX tumors and 3-D Gelfoam histoculture was developed.