RT Journal Article SR Electronic T1 The Biological Role of the Long Non-coding RNA LINK-A in Ovarian Carcinoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6677 OP 6684 DO 10.21873/anticanres.14691 VO 40 IS 12 A1 FILIPPOV-LEVY, NATALIE A1 DAVIDSON, BEN A1 REICH, REUVEN YR 2020 UL http://ar.iiarjournals.org/content/40/12/6677.abstract AB Aim: To analyze the biological role of the long non-coding RNA LINK-A. Materials and Methods: An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro. Results: OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation. Conclusion: Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.