<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">YOKOYAMA, KEIKO</style></author><author><style face="normal" font="default" size="100%">HASHIBA, KANA</style></author><author><style face="normal" font="default" size="100%">WAKABAYASHI, HIDETSUGU</style></author><author><style face="normal" font="default" size="100%">HASHIMOTO, KEN</style></author><author><style face="normal" font="default" size="100%">SATOH, KAZUE</style></author><author><style face="normal" font="default" size="100%">KURIHARA, TERUO</style></author><author><style face="normal" font="default" size="100%">MOTOHASHI, NOBORU</style></author><author><style face="normal" font="default" size="100%">SAKAGAMI, HIROSHI</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Inhibition of LPS-stimulated NO Production in Mouse Macrophage-like Cells by Tropolones</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004-11-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">3917-3922</style></pages><volume><style face="normal" font="default" size="100%">24</style></volume><issue><style face="normal" font="default" size="100%">6</style></issue><abstract><style  face="normal" font="default" size="100%">We investigated the effect of 27 tropolones on nitric oxide (NO) production by mouse macrophage-like Raw 264.7 cells. All of these compounds failed to stimulate the Raw 264.7 cells to produce detectable amounts of NO, but inhibited NO production by lipopolysaccharide (LPS)-activated Raw 264.7 cells to various extents. Generally, the ability of tropolones to inhibit LPS-stimulated NO production was inversely related to their cytotoxic activity. Western blot and RT-PCR analyses demonstrated that the most active compound, 2,4-dibromo-7-methoxytropone [21], significantly reduced both the intracellular concentration of iNOS protein and the expression of iNOS mRNA. ESR spectroscopy showed that [21] did not produce radicals under alkaline condition, nor scavenged NO, produced by NOC-7. These data suggested that the inhibitory effect of [21] on NO production might be generated via the inhibition of iNOS expression, rather than a radical-mediated mechanism. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved</style></abstract></record></records></xml>