RT Journal Article
SR Electronic
T1 Single Nucleotide Polymorphisms in <em>MMP1</em> and <em>MMP3</em> Gene Promoters as Risk Factor in Head and Neck Squamous Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2021
OP 2026
VO 24
IS 3B
A1 FRANCK ZINZINDOHOUÉ
A1 HÉLENE BLONS
A1 STÉPHANE HANS
A1 MARIE-ANNE LORIOT
A1 ANNE-MARIE HOULLIER
A1 DANIEL BRASNU
A1 OLLIVIER LACCOURREYE
A1 DAVID-ALEXANDRE TREGOUET
A1 ISABELLE STUCKER
A1 PIERRE LAURENT-PUIG
YR 2004
UL http://ar.iiarjournals.org/content/24/3B/2021.abstract
AB Matrix metalloproteinase (MMP) 1 and 3 genes play an important role in initiating tumor growth and promoting cell spread. Since MMP1 and MMP3 transcription levels can be modulated by promoter polymorphism, we investigated the impact of different genotypes on the occurrence of head and neck cancer in a Caucasian case control study. DNA was extracted from 126 male head and neck cancer patients and from 249 male hospitalized-based controls. Genotyping was carried out using PCR multiplex allowing the co-amplification of MMP1-1607 bp and MMP3-1171 bp polymorphisms. PCR products were separated on a capillary electrophoresis. The MMP1-2G and the MMP3-6A allele frequencies were significantly lower in cases than in controls. In particular homozygous 2G/2G individuals were at lower risk of cancer than the 1G/1G carriers (OR= 0.37 95%CI [0.19-0.71], p=0.003). MMP1 and MMP3 polymorphisms were in moderate linkage disequilibrium in cases and controls (D'=0.41 and D'=0.46). Haplotype frequencies distribution derived from these 2 polymorphisms was significantly different between cases and controls (p=0.01). The haplotype analysis suggested an implication of both MMP1 and MMP3 polymorphisms in the head and neck squamous cell carcinoma susceptibility. Indeed, the presence of the MMP1-2G and MMP3-6A alleles seemed to be associated with decreased risk of head and neck squamous cell carcinoma but mainly when they were carried by the same haplotype. By comparison to the 1G-5A haplotype, the 2G-6A haplotype was associated with a lower risk of head and neck squamous cell carcinoma (OR= 0.52 95%CI [0.34-0.80], p=0.003). Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved