TY - JOUR T1 - Dose-dependent Changes After Proton and Photon Irradiation in a Zebrafish Model JF - Anticancer Research JO - Anticancer Res SP - 6123 LP - 6135 DO - 10.21873/anticanres.14633 VL - 40 IS - 11 AU - SZILVIA BRUNNER AU - TÜNDE TŐKÉS AU - EMÍLIA RITA SZABÓ AU - RÓBERT POLANEK AU - IMRE ZOLTÁN SZABÓ AU - ZITA REISZ AU - BARBARA KONCZNÉ GUBÁN AU - ANDRÁS LAJOS SZIJÁRTÓ AU - MICHAEL BRAND AU - STEFAN HANS AU - LEONHARD KARSCH AU - ELISABETH LESSMANN AU - JÖRG PAWELKE AU - MICHAEL SCHÜRER AU - ELKE BEYREUTHER AU - KATALIN HIDEGHÉTY Y1 - 2020/11/01 UR - http://ar.iiarjournals.org/content/40/11/6123.abstract N2 - Background/Aim: The importance of hadron therapy in the cancer management is growing. We aimed to refine the biological effect detection using a vertebrate model. Materials and Methods: Embryos at 24 and 72 h postfertilization were irradiated at the entrance plateau and the mid spread-out Bragg peak of a 150 MeV proton beam and with reference photons. Radiation-induced DNA double-strand breaks (DSB) and histopathological changes of the eye, muscles and brain were evaluated; deterioration of specific organs (eye, yolk sac, body) was measured. Results: More and longer-lasting DSBs occurred in eye and muscle cells due to proton versus photon beams, albeit in different numbers. Edema, necrosis and tissue disorganization, (especially in the eye) were observed. Dose-dependent morphological deteriorations were detected at ≥10 Gy dose levels, with relative biological effectiveness between 0.99±0.07 (length) and 1.12±0.19 (eye). Conclusion: Quantitative assessment of radiation induced changes in zebrafish embryos proved to be beneficial for the radiobiological characterization of proton beams. ER -