RT Journal Article
SR Electronic
T1 Dose-dependent Changes After Proton and Photon Irradiation in a Zebrafish Model
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6123
OP 6135
DO 10.21873/anticanres.14633
VO 40
IS 11
A1 SZILVIA BRUNNER
A1 TÜNDE TŐKÉS
A1 EMÍLIA RITA SZABÓ
A1 RÓBERT POLANEK
A1 IMRE ZOLTÁN SZABÓ
A1 ZITA REISZ
A1 BARBARA KONCZNÉ GUBÁN
A1 ANDRÁS LAJOS SZIJÁRTÓ
A1 MICHAEL BRAND
A1 STEFAN HANS
A1 LEONHARD KARSCH
A1 ELISABETH LESSMANN
A1 JÖRG PAWELKE
A1 MICHAEL SCHÜRER
A1 ELKE BEYREUTHER
A1 KATALIN HIDEGHÉTY
YR 2020
UL http://ar.iiarjournals.org/content/40/11/6123.abstract
AB Background/Aim: The importance of hadron therapy in the cancer management is growing. We aimed to refine the biological effect detection using a vertebrate model. Materials and Methods: Embryos at 24 and 72 h postfertilization were irradiated at the entrance plateau and the mid spread-out Bragg peak of a 150 MeV proton beam and with reference photons. Radiation-induced DNA double-strand breaks (DSB) and histopathological changes of the eye, muscles and brain were evaluated; deterioration of specific organs (eye, yolk sac, body) was measured. Results: More and longer-lasting DSBs occurred in eye and muscle cells due to proton versus photon beams, albeit in different numbers. Edema, necrosis and tissue disorganization, (especially in the eye) were observed. Dose-dependent morphological deteriorations were detected at ≥10 Gy dose levels, with relative biological effectiveness between 0.99±0.07 (length) and 1.12±0.19 (eye). Conclusion: Quantitative assessment of radiation induced changes in zebrafish embryos proved to be beneficial for the radiobiological characterization of proton beams.