%0 Journal Article %A SZILVIA BRUNNER %A TÜNDE TŐKÉS %A EMÍLIA RITA SZABÓ %A RÓBERT POLANEK %A IMRE ZOLTÁN SZABÓ %A ZITA REISZ %A BARBARA KONCZNÉ GUBÁN %A ANDRÁS LAJOS SZIJÁRTÓ %A MICHAEL BRAND %A STEFAN HANS %A LEONHARD KARSCH %A ELISABETH LESSMANN %A JÖRG PAWELKE %A MICHAEL SCHÜRER %A ELKE BEYREUTHER %A KATALIN HIDEGHÉTY %T Dose-dependent Changes After Proton and Photon Irradiation in a Zebrafish Model %D 2020 %R 10.21873/anticanres.14633 %J Anticancer Research %P 6123-6135 %V 40 %N 11 %X Background/Aim: The importance of hadron therapy in the cancer management is growing. We aimed to refine the biological effect detection using a vertebrate model. Materials and Methods: Embryos at 24 and 72 h postfertilization were irradiated at the entrance plateau and the mid spread-out Bragg peak of a 150 MeV proton beam and with reference photons. Radiation-induced DNA double-strand breaks (DSB) and histopathological changes of the eye, muscles and brain were evaluated; deterioration of specific organs (eye, yolk sac, body) was measured. Results: More and longer-lasting DSBs occurred in eye and muscle cells due to proton versus photon beams, albeit in different numbers. Edema, necrosis and tissue disorganization, (especially in the eye) were observed. Dose-dependent morphological deteriorations were detected at ≥10 Gy dose levels, with relative biological effectiveness between 0.99±0.07 (length) and 1.12±0.19 (eye). Conclusion: Quantitative assessment of radiation induced changes in zebrafish embryos proved to be beneficial for the radiobiological characterization of proton beams. %U https://ar.iiarjournals.org/content/anticanres/40/11/6123.full.pdf