PT  - JOURNAL ARTICLE
AU  - KORBYN J.V. DAHLQUIST
AU  - LAURA C. VOTH
AU  - AMANDA J. FEE
AU  - ANGELA K. STOECKMAN
TI  - An Autocrine Role for CXCL1 in Progression of Hepatocellular Carcinoma
AID  - 10.21873/anticanres.14628
DP  - 2020 Nov 01
TA  - Anticancer Research
PG  - 6075--6081
VI  - 40
IP  - 11
4099  - http://ar.iiarjournals.org/content/40/11/6075.short
4100  - http://ar.iiarjournals.org/content/40/11/6075.full
SO  - Anticancer Res2020 Nov 01; 40
AB  - Background: One of the most prevalent causes of cancer fatalities is hepatocellular carcinoma (HCC), which has been linked to metabolic syndrome. Circulating levels of the saturated fatty acid palmitate are elevated in metabolic syndrome and lead to cellular stress. Materials and Methods: Using enzyme-linked immunosorbent assay, flow cytometry, and migration assays, we characterized the response of rat hepatoma cells to palmitate treatment. Results: We detected a 60% increase in secretion of C-X-C motif ligand 1 (CXCL1) which was dose-dependent and coincided with apoptosis. We measured expression of C-X-C motif chemokine receptor 2 (CXCR2) and observed a 4.5-fold increase on apoptotic hepatoma cells. Furthermore, we assayed migration of hepatoma cells and saw a 2-fold increase in the number of migrating cells towards CXCL1. Conclusion: These findings suggest that HCC cells secrete CXCL1 in response to metabolic syndrome signals and may promote the progression of cancer through apoptosis recovery or metastasis.