RT Journal Article
SR Electronic
T1 Involvement of the MicroRNA-1-LITAF Axis in Gastric Cancer Cell Growth and Invasion
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6247
OP 6256
DO 10.21873/anticanres.14645
VO 40
IS 11
A1 YEN-CHIH CHEN
A1 CHAO-CHUAN WU
A1 YA-TING TU
A1 YI-RU CHEN
A1 MING-CHENG LEE
A1 KUO-WANG TSAI
YR 2020
UL http://ar.iiarjournals.org/content/40/11/6247.abstract
AB Background/Aim: Lipopolysaccharide-induced tumor necrosis factor alpha factor (LITAF) has been identified as a tumor suppressor in human cancers. Present study, we assessed biological role of LITAF in human gastric cancer. Materials and Methods: The clinical impacts of LITAF expression were assessed in gastric cancer using public databases. The biological role of LITAF was assessed in gastric cancer cells using siLITAF transfection. Results: High LITAF expression was correlated well with worse prognosis, including pathological stage (p=0.034) and pathological T stage (p=0.047), as well as with shorter survival. Herein, we present a novel finding that miR-1-3p could inhibit LITAF expression by directly binding to the 3’-untranslated region of LITAF mRNA. Cell functional assays revealed that LITAF knockdown could significantly suppress gastric cancer growth and motility. Conclusion: High LITAF expression resulting from low miR-1-3p expression is a biomarker for poor prognosis or therapeutic targets in gastric cancer.