TY - JOUR T1 - Synthetic Betulin Derivatives Inhibit Growth of Glioma Cells <em>In Vitro</em> JF - Anticancer Research JO - Anticancer Res SP - 6151 LP - 6158 DO - 10.21873/anticanres.14635 VL - 40 IS - 11 AU - SYLWIA K. KRÓL AU - EWA BĘBENEK AU - ADRIANNA SŁAWIŃSKA-BRYCH AU - MAGDALENA DMOSZYŃSKA-GRANICZKA AU - STANISŁAW BORYCZKA AU - ANDRZEJ STEPULAK Y1 - 2020/11/01 UR - http://ar.iiarjournals.org/content/40/11/6151.abstract N2 - Background/Aim: Glioma is the most malignant tumour of the human brain still lacking effective treatment modalities. Betulin, a pentacyclic triterpene abundantly found in the birch bark, has been shown to demonstrate interesting anti-cancer activity towards many cancer cells. We determined the effects of acetylenic synthetic betulin derivatives (ASBDs) as anti-tumour agents on glioma cells in vitro. Materials and Methods: T98G and C6 glioma cell viability and proliferation were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and BrdU (bromo deoxyuridine) test, respectively. Cell-cycle progression and induction of apoptosis were investigated with flow cytometry. Results: ASBDs significantly decreased glioma cell viability/survival and inhibited proliferation in a dose-dependent manner in vitro. Moreover, ASBDs were more cytotoxic than clinically used chemotherapeutics – temozolomide and cisplatin. Conclusion: ASBDs may be considered for further study as potent anti-tumour agents in glioma treatment. ER -