RT Journal Article
SR Electronic
T1 Melatonin Exerts Anticancer Effects in Human Tongue Squamous Cell Carcinoma Cells by Promoting Autophagy
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6295
OP 6303
DO 10.21873/anticanres.14650
VO 40
IS 11
A1 EUI-SUK SUNG
A1 JOO-YOUNG KIM
A1 YONG TAE AHN
A1 IL-WOO LEE
A1 SEONG-WOOK CHOI
A1 HYO-BEOM JANG
A1 JIN-CHOON LEE
A1 WON G. AN
YR 2020
UL http://ar.iiarjournals.org/content/40/11/6295.abstract
AB Background/Aim: The global prevalence of head and neck squamous cell carcinoma (HNSCC) remains high, and its prognosis poor. We investigated the anticancer effects of melatonin in human tongue squamous cell carcinoma cells (SCC-25) and its mechanisms of action. Materials and Methods: MTT assay was used to determine cell viability. To assess the effects of melatonin on SCC-25 cell metastasis, we conducted cell formation, wound healing, transwell migration and invasion assay. Western blot analysis was performed to measure the levels of autophage marker proteins. Results: We found that melatonin treatment significantly reduced the viability and colony formation ability of SCC-25 cells, impairing cell migration and invasion. Western blotting assay revealed that melatonin increased the levels of autophagy markers, such as LC-3B and Beclin-1. Consequently, melatonin induces autophage in SCC-25 cells. Conclusion: Melatonin may be a promising anticancer agent for the treatment of human tongue squamous cell carcinoma.