PT  - JOURNAL ARTICLE
AU  - CHRISTOPH PAUTKE
AU  - MATTHIAS SCHIEKER
AU  - THOMAS TISCHER
AU  - ANDREAS KOLK
AU  - PETER NETH
AU  - WOLF MUTSCHLER
AU  - STEFAN MILZ
TI  - Characterization of Osteosarcoma Cell Lines MG-63, Saos-2 and U-2 OS in Comparison to Human Osteoblasts
DP  - 2004 Nov 01
TA  - Anticancer Research
PG  - 3743--3748
VI  - 24
IP  - 6
4099  - http://ar.iiarjournals.org/content/24/6/3743.short
4100  - http://ar.iiarjournals.org/content/24/6/3743.full
SO  - Anticancer Res2004 Nov 01; 24
AB  - Background: Osteosarcomas are malignant bone tumors consisting of cells with abnormal cellular functions. Although osteosarcoma-derived cells are commonly used for osteoblastic models, the molecular composition of the osteosarcoma extracellular matrix (ECM) is not well characterized. Materials and Methods: We compared three osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS) with normal human osteoblasts by immunocytochemistry. Cellular characteristics were assessed by morphometric analysis and proliferation kinetics. Results: All investigated osteosarcoma cell lines exhibited very heterogeneous labelling profiles and each differed significantly from that of normal osteoblasts. Saos-2 cells revealed the most mature osteoblastic labelling profile while U-2 OS cells were negative for most of the investigated osteoblastic markers. Conclusion: We conclude that each osteosarcoma cell line exhibits a characteristic labelling profile and thus produces a differently composed extracellular matrix. This can be used in attempts to better characterize osteosarcoma, a as well as for their diagnosis. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved