RT Journal Article
SR Electronic
T1 Protein Profiling of Extracellular Vesicles Associated With Cisplatin Resistance in Lung Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5509
OP 5516
DO 10.21873/anticanres.14563
VO 40
IS 10
A1 HELIER BALBINOTTI
A1 NATHAN A. CADORE
A1 CRISTINE S. DUTRA
A1 EDILEUZA D. DA SILVA
A1 HENRIQUE B. FERREIRA
A1 ARNALDO ZAHA
A1 KARINA M. MONTEIRO
YR 2020
UL http://ar.iiarjournals.org/content/40/10/5509.abstract
AB Background/Aim: Extracellular vesicles (EVs) can mediate drug resistance within the tumor microenvironment by delivering bioactive molecules, including proteins. Here, we performed a comparative proteomic analysis of EVs secreted by A549 lung cancer cells and their cisplatin-resistant counterparts in order to identify proteins involved in drug resistance. Materials and Methods: Cells were co-cultivated using a transwell system to evaluate EV exchange. EVs were isolated by ultracentrifugation and analyzed using microscopy and nanoparticle tracking. EV proteome was analyzed by mass spectrometry. Results: EV-mediated communication was observed between co-cultured A549 and A549/CDDP cells. EVs isolated from both cells were mainly exosome-like structures. Extracellular matrix components, cell adhesion proteins, complement factors, histones, proteasome subunits and membrane transporters were found enriched in the EVs released by cisplatin-resistant cells. Conclusion: Proteins identified in this work may have a relevant role in modulating the chemosensitivity of the recipient cells and could represent useful biomarkers to monitor cisplatin response in lung cancer.