@article {BALBINOTTI5509, author = {HELIER BALBINOTTI and NATHAN A. CADORE and CRISTINE S. DUTRA and EDILEUZA D. DA SILVA and HENRIQUE B. FERREIRA and ARNALDO ZAHA and KARINA M. MONTEIRO}, title = {Protein Profiling of Extracellular Vesicles Associated With Cisplatin Resistance in Lung Cancer}, volume = {40}, number = {10}, pages = {5509--5516}, year = {2020}, doi = {10.21873/anticanres.14563}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Extracellular vesicles (EVs) can mediate drug resistance within the tumor microenvironment by delivering bioactive molecules, including proteins. Here, we performed a comparative proteomic analysis of EVs secreted by A549 lung cancer cells and their cisplatin-resistant counterparts in order to identify proteins involved in drug resistance. Materials and Methods: Cells were co-cultivated using a transwell system to evaluate EV exchange. EVs were isolated by ultracentrifugation and analyzed using microscopy and nanoparticle tracking. EV proteome was analyzed by mass spectrometry. Results: EV-mediated communication was observed between co-cultured A549 and A549/CDDP cells. EVs isolated from both cells were mainly exosome-like structures. Extracellular matrix components, cell adhesion proteins, complement factors, histones, proteasome subunits and membrane transporters were found enriched in the EVs released by cisplatin-resistant cells. Conclusion: Proteins identified in this work may have a relevant role in modulating the chemosensitivity of the recipient cells and could represent useful biomarkers to monitor cisplatin response in lung cancer.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/40/10/5509}, eprint = {https://ar.iiarjournals.org/content/40/10/5509.full.pdf}, journal = {Anticancer Research} }