@article {K{\"O}DITZ5539, author = {BARBARA K{\"O}DITZ and JOCHEN W.U. FRIES and HEIKE G{\"O}BEL and PIA PAFFENHOLZ and KONSTANTIN RICHTER and AXEL HEIDENREICH and MELANIE VON BRANDENSTEIN}, title = {Mxi-2 Dependent Regulation of p53 in Prostate Cancer}, volume = {40}, number = {10}, pages = {5539--5544}, year = {2020}, doi = {10.21873/anticanres.14566}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Endothelin-1 (ET-1) is overexpressed in many types of cancer, inhibiting the release of the microRNA 15a (miR-15a) and inducing the production of Mxi-2. Our aim was to identify a molecular complex regulating p53 activity in prostate cancer (PCa). Materials and Methods: DU145 cells were treated with ET-1, MAPK p38 inhibitor, Endothelin A receptor inhibitor (ETAR inhibitor) and Endothelin B receptor inhibitor (ETBR inhibitor). Extracts were analysed using Western Blot, immunoprecipitation and qRT{\textendash}PCR. Furthermore, prostate cancer patient samples were analysed using qRT{\textendash}PCR and ELISA. Results: The hypothesised molecular complex was identified, with miR-15a, microRNA 1285 (miR-1285) and Mxi-2 levels up-regulated in patients in relation to increasing aggressiveness of PCa. Conclusion: A complex composed of Argonaut 2 (Ago2)/Mxi-2/miR-1285 is involved in PCa. The expression of Mxi-2 correlates with increasing PCa aggressiveness and might be used as a non-invasive marker for the diagnosis and progression of PCa.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/40/10/5539}, eprint = {https://ar.iiarjournals.org/content/40/10/5539.full.pdf}, journal = {Anticancer Research} }