TY - JOUR T1 - A Novel System to Detect Circulating Tumor Cells Using Two Different Size-selective Microfilters JF - Anticancer Research JO - Anticancer Res SP - 5577 LP - 5582 DO - 10.21873/anticanres.14570 VL - 40 IS - 10 AU - TOMOAKI SONODA AU - NORIKO YANAGITANI AU - KANAKO SUGA AU - TAKAHIRO YOSHIZAWA AU - SHINGO NISHIKAWA AU - SATORU KITAZONO AU - ATSUSHI HORIIKE AU - KIYOTAKA SHIBA AU - TAMOTSU ISHIZUKA AU - MAKOTO NISHIO AU - SATOSHI MATSUSAKA Y1 - 2020/10/01 UR - http://ar.iiarjournals.org/content/40/10/5577.abstract N2 - Background/Aim: Clusters of circulating tumor cells (CTCs) increase metastatic potential compared to single CTC. However, conventional technologies have been unable to generate an accurate analysis of single and cluster CTCs in the peripheral blood. We propose an effective strategy to detect and isolate both single and cluster CTCs using two size-selective microfilters. Materials and Methods: Five ml of whole blood were collected from 10 patients with epidermal growth factor receptor mutation-positive non-small cell lung cancer. Single and cluster CTCs were identified using precision microfiltration membranes with two distinct pore sizes together with anti-EpCAM antibody labeling. Results: Single and cluster CTCs were detected by simultaneously using two size-selective microfilters. The EGFR-L858R mutation was detected in the DNA from cells captured using both microfilters. Conclusion: Our method can be used to detect and isolate single and cluster CTCs in the whole blood and may facilitate the development of a liquid biopsy strategy. ER -