PT  - JOURNAL ARTICLE
AU  - RAJENDRA GHARBARAN
AU  - ENYUAN SHANG
AU  - ONYEKWERE ONWUMERE
AU  - NAOMI CODRINGTON
AU  - EVANGELINA DANKWA SARPONG
AU  - STEPHEN REDENTI
TI  - Luteolin Induces Cytotoxicity in Mix Cellularity Classical Hodgkin's Lymphoma <em>via</em> Caspase Activated-cell Death
AID  - 10.21873/anticanres.14493
DP  - 2020 Sep 01
TA  - Anticancer Research
PG  - 4907--4912
VI  - 40
IP  - 9
4099  - http://ar.iiarjournals.org/content/40/9/4907.short
4100  - http://ar.iiarjournals.org/content/40/9/4907.full
SO  - Anticancer Res2020 Sep 01; 40
AB  - Background/Aim: We investigated the effects of luteolin (LUT) on classical Hodgkin's lymphoma (cHL), since such studies in malignant lymphomas are lacking. Materials and Methods: Effect of LUT on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry on trypan blue-exclusion assay. Cell death was investigated with acridine orange/ethidium bromide live-dead assay, propidium iodide (PI) flow cytometry, and Annexin-V-PI microscopy. Caspase activation was studied using CellEvent Caspase-3/7 Green detection reagent. High resolution immunofluorescence microscopy was used to detect cleaved-PARP-1. Results: LUT induced a dose-dependent decrease in the growth of KMH2 and L428 cells, cellular models of mix-cellularity (MC) and nodular sclerosis (NS) cHL, respectively. However, LUT induced cell death only in KMH2, at a higher concentration, and this was associated with caspase activation and cleaved PARP-1. Conclusion: LUT induces cytotoxicity in the MC-cHL cellular model KMH2 via caspase activation.