TY - JOUR T1 - Inhibitory Effect of Eicosapentaenoic Acid on the Migration of the Esophageal Squamous Cell Carcinoma Cell Line TE-1 JF - Anticancer Research JO - Anticancer Res SP - 5043 LP - 5048 DO - 10.21873/anticanres.14507 VL - 40 IS - 9 AU - HISAKO KUBOTA AU - TOSHIHIRO HIRAI AU - AKIRA YAMAUCHI AU - AYAKO OGO AU - HIDEO MATSUMOTO AU - TOMIO UENO Y1 - 2020/09/01 UR - http://ar.iiarjournals.org/content/40/9/5043.abstract N2 - Background/Aim: Eicosapentaenoic acid (EPA) inhibits NF-ĸB activation and IL-6 production in TE-1 esophageal cancer cells. NF-ĸB is related to cancer cell migration. The aim of this study is to evaluate whether EPA has a metastasis suppressing effect. Herein, we investigated EPA-treated TE-1 cell migration using TAXIScan. Materials and Methods: EZ-TAXIScan® was used to verify whether EPA inhibits cancer cell chemotaxis. Results: Using 50% fetal bovine serum (chemoattractant) without EPA (positive control), average velocity was 0.306±0.084 μm/min compared to 0.162±0.067 μm/min without chemoattraction (negative control). Directionalities of positive and negative controls were 1.039±0.152 and 0.488±0.251 radians, respectively, indicating a significant increase in migration of the positive control compared to that of the negative control. Average velocities were 0.306±0.084 (no EPA), 0.288±0.078 (100 μM EPA), and 0.240±0.054 200 μM (EPA) μm/min, indicating that EPA reduced velocity dose-dependently. Average directionalities were 1.039±0.152 (no EPA), 0.967±0.164 (100 μM EPA), and 0.901±0.146 (200 μM EPA) radians, indicating that EPA also inhibited directionality dose-dependently. Conclusion: EPA suppresses directional migration of TE-1 cells. ER -