TY - JOUR T1 - Effect of Phosphoribosyltransferase Down-regulation on Malignant Glioma Cell Characteristics JF - Anticancer Research JO - Anticancer Res SP - 4895 LP - 4905 DO - 10.21873/anticanres.14492 VL - 40 IS - 9 AU - MINORI KAMADA AU - KEIICHI IKEDA AU - YOSHINOBU MANOME Y1 - 2020/09/01 UR - http://ar.iiarjournals.org/content/40/9/4895.abstract N2 - Background/Aim: Nicotinamide phosphoribosyl-transferase (NAMPT) is a rate-limiting enzyme in the pathway synthesizing nicotinamide adenine dinucleotide (NAD (+)) from nicotinamide (NAM). Glioma tissues exhibit up-regulated NAMPT expression associated with a poor prognosis of patients. To determine if NAMPT can be a molecular therapeutic target, we investigated the effects of short hairpin RNA (shRNA)-mediated NAMPT down-regulation. Materials and Methods: We designed shRNA to NAMPT and transfected to T98G cells. The characteristics of these cells were analyzed. Results: The NAMPT shRNA-transfected cells exhibited delayed cell growth. However, there was no difference in the increase of sensitivity to temozolomide (TMZ) or X-ray irradiation between the NAMPT and scramble shRNA-transfected cells. The expression of NAMPT in the NAMPT shRNA-transfected cells increased with cell passage. Additionally, the shRNA-mediated transfection was associated with enhanced expression of quinolinic acid phosphoribo-syltransferase (QPRT). Conclusion: shRNA-mediated NAMPT down-regulation may not decrease the NADt to a sufficient level to increase TMZ/radiation sensitivity. ER -