%0 Journal Article %A YING-HAO HAN %A WEI-LONG LI %A MEI-HUA JIN %A YING-HUA JIN %A YOUNG-QING ZHANG %A LING-ZU KONG %A LI-YUN YU %A JIN WON HYUN %A JEONGWOO KWON %A HU-NAN SUN %A TAEHO KWON %T Peroxiredoxin II Inhibits Alcohol-induced Apoptosis in L02 Hepatocytes Through AKT/β-Catenin Signaling Pathway %D 2020 %R 10.21873/anticanres.14454 %J Anticancer Research %P 4491-4504 %V 40 %N 8 %X Background: Peroxiredoxin II (PRDX2) performs unique roles in cells. It can reduce peroxides through cysteine residues, and helps prevent the effects of oxidative stress on cells. It is closely related to the occurrence and development of various diseases, especially alcoholic liver injury and even liver cancer. The metabolism of alcohol in hepatocytes leads to the increase in the levels of reactive oxygen species (ROS), oxidative stress, injury, and apoptosis. Therefore, this study focused on the investigating the protection conferred by PRDX2 against alcohol-induced apoptosis of hepatocytes. Materials and Methods: PRDX2 inhibition of alcohol-induced apoptosis in L02 hepatocytes was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, fluorescence microscopy, flow cytometry, western blotting and hematoxylin and eosin staining. Results: The results showed that the levels of reactive oxygen species, protein kinase B, β-catenin, B-cell lymphoma-2 (BCL2), BCL-XL, BCL2-associated X, cleaved caspase-3, and cleaved poly (ADP-ribose) polymerase in PRDX2-silenced cells were increased significantly after the treatment of cells with ethanol. Similar results were obtained in an in vivo Prdx2-knockout mouse model of alcoholic liver injury. Therefore, PRDX2 may regulate the phosphorylation of the AKT signal protein by eliminating reactive oxygen species from cells, and it inhibits the downstream mitochondria-dependent apoptosis pathway, and, thereby, the apoptosis of cells. Conclusion: Thus, PRDX2 may be a potential molecular target for the prevention and treatment of alcoholic liver injury. %U https://ar.iiarjournals.org/content/anticanres/40/8/4491.full.pdf