RT Journal Article
SR Electronic
T1 Retinoic Acid Enhances Histamine Content and H1 Receptor Expression in Human Neuroblastoma Cell Line Paju
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1657
OP 1664
VO 24
IS 3A
A1 H. HEGYESI
A1 ZS. DARVAS
A1 V. LASZLO
A1 Z. POS
A1 É. PALLINGER
A1 K. HIRSCHBERG
A1 P. KOVACS
A1 A. FALUS
YR 2004
UL http://ar.iiarjournals.org/content/24/3A/1657.abstract
AB Background: A human neuroblastoma cell line (Paju) was induced by retinoic acid (RA) to differentiate into neuron-like cells. Materials and Methods: We studied the expression and the possible role of histamine receptors H1 and H2 in retinoic-acid mediated differentiation by semiquantitative RT-PCR. We studied the effect of exogeneously added RA on the morphological change of the human neuroblastoma cell line and the differentiation was followed by vimentine, glial fibrillary acidic protein (GFAP) and neurofilament (NF) immunostaining. We monitored the change of the histidine decarboxylase (HDC) expression and the histamine content during the RA treatment by immunoblot and flow cytometry methods. Results: Our data showed that H1 and H2 histamine receptors are present on Paju cells. Ten nM RA markedly increased the H1 receptor expression of these cells, while the H2 expression was unchanged. Conclusion: In the RA-treated Paju cells, the histamine content increased compared to the untreated cells, suggesting that neuroblastoma-derived histamine is involved in the regulation of RA-induced in vitro differentiation by H1 receptors. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved