RT Journal Article
SR Electronic
T1 Granulocyte/Macrophage Colony-stimulating Factor and Interleukin-4-induced Dendritic Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1609
OP 1616
VO 24
IS 3A
A1 HAJIME HIKINO
A1 KEIZO KASONO
A1 MASAKI KANZAKI
A1 TOSHIHIRO KAI
A1 FUMIO KONISHI
A1 MASANOBU KAWAKAMI
YR 2004
UL http://ar.iiarjournals.org/content/24/3A/1609.abstract
AB Background: We investigated whether GM-CSF/IL-4 is the most efficient cytokine combination for differentiating dendritic cells (DC) in terms of its ability to elicit an antitumor immune response. Materials and Methods: Two experimental models were examined: C57BL/6 mice bearing MC38 cells and Balb/c mice bearing cachexia-inducible Colon-26 cells. After immunization with DC pulsed with whole tumor cell lysate, tumors were inoculated into the subcutis. Results: C57BL/6 mice immunized with lysate-pulsed DC effectively rejected the MC38 challenge and detectable MC38-specific cytotoxic lymphocytes (CTL) were observed. However, even those groups immunized with lysate-pulsed DC exhibited no protective immunity against Colon-26 challenge in Balb/c mice. Unexpectedly, mice inoculated with lysate-unpulsed DC showed an acceleration of cachectic progression (p=0.031) compared to control mice. Conclusion: We speculate that GMCSF/IL-4-induced DC promotes Th2-dominated immunity in Balb/c mice. Consideration might be given to which combination of cytokines is appropriate for the ex vivo differentiation of DC in tumor immunotherapy. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved