RT Journal Article
SR Electronic
T1 Human Herpesvirus Type 8 Genotypes in Iatrogenic, Classic and AIDS-associated Kaposi's Sarcoma from Greece
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1597
OP 1602
VO 24
IS 3A
A1 M. GAZOULI
A1 I. PAPACONSTANTINOU
A1 G. ZAVOS
A1 V. METAXA-MARIATOU
A1 G. NASIOULAS
A1 J. BOLETIS
A1 P. ARAPADONI-DADIOTI
A1 K. GIASLAKIOTIS
A1 A. ZOGRAFIDIS
A1 A. KOSTAKIS
YR 2004
UL http://ar.iiarjournals.org/content/24/3A/1597.abstract
AB Background: Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) is consistently found in almost all observed Kaposi's sarcomas (KS), whether AIDS-associated, iatrogenic or classic. To our knowledge no data are available on the genetic polymorphism of HHV-8 from Greece. We report the study of 15 renal transplant recipients with KS, 5 with AIDS-associated KS, 11 with classic KS and 60 healthy individuals from Greece. Materials and Methods: Polymerase chain reaction (PCR) was carried out on DNA extracted from peripheral-blood mononuclear cells (PBMC) or KS cutaneous biopsies, using specific primers for the HHV-8, KS330 fragment from ORF-26 (233 bp) and the highly variable region (VR1) from ORF-K1 (363 bp). Results: HHV-8 DNA was detected in 30 out of 31 (97%) KS cases, regardless of their clinico-pathological subtype and in 10 out of 60 (16.7%) healthy individuals. Sequencing of the ORF26 fragment demonstrated that the 40 HHV-8 strains were of the A and C sub-types. Furthermore, sequencing of the ORF-K1 showed that these HHV-8 strains of Greek origin were of the A1, A4, C1 or C3 sub-type. Conclusion: Our findings imply a possible link of the C3 subtype of HHV-8 in renal transplant-related KS cases (iatrogenic KS) in Greece, a link of the A4 subtype in AIDS-associated KS cases and a potential involvement of the A1 subtype in Greek classic KS incidences, as HHV-8 strains among healthy individual tested belong to the C1, C3 or A1 subtypes. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved