RT Journal Article
SR Electronic
T1 Ultrastructure of Sarcoma 180 Cells After Ultrasound Irradiation in the Presence of Sparfloxacin
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1553
OP 1560
VO 24
IS 3A
A1 HUANG, DEQING
A1 OKADA, KYOJI
A1 KOMORI, CHIYO
A1 ITOI, EIJI
A1 KAWAMURA, KOICHI
A1 SUZUKI, TOSHIO
YR 2004
UL http://ar.iiarjournals.org/content/24/3A/1553.abstract
AB Background: Sonodynamic synergistic antitumor effects have been well described to date, but detailed ultrastructural studies in this field are still lacking. Materials and Methods: A suspension of sarcoma 180 cells was exposed to ultrasound (US) in the presence of sparfloxacin (SPFX), one of the new quinolone antibiotics, at 2 W (0.64 W/cm2) for 30 seconds. The antitumor effect was evaluated by the survival rate of cells and cell morphology by scanning and transmission electron microscopy. Results: After US irradiation, the survival rate of tumor cells in the SPFX-added group was 30.9%, significantly lower than 78.7% in the control group (p=0.0002). Most cells in the control group were spherical, but in the SPFX-added group the cells were aspherical. Cell membranes were often ruptured and numerous pores of various sizes were observed. Some cells were totally disintegrated. Conclusion: The most characteristic changes of the synergistic antitumor activities of US and SPFX were on the cell membrane. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved