TY - JOUR T1 - Ultrastructure of Sarcoma 180 Cells After Ultrasound Irradiation in the Presence of Sparfloxacin JF - Anticancer Research JO - Anticancer Res SP - 1553 LP - 1560 VL - 24 IS - 3A AU - DEQING HUANG AU - KYOJI OKADA AU - CHIYO KOMORI AU - EIJI ITOI AU - KOICHI KAWAMURA AU - TOSHIO SUZUKI Y1 - 2004/05/01 UR - http://ar.iiarjournals.org/content/24/3A/1553.abstract N2 - Background: Sonodynamic synergistic antitumor effects have been well described to date, but detailed ultrastructural studies in this field are still lacking. Materials and Methods: A suspension of sarcoma 180 cells was exposed to ultrasound (US) in the presence of sparfloxacin (SPFX), one of the new quinolone antibiotics, at 2 W (0.64 W/cm2) for 30 seconds. The antitumor effect was evaluated by the survival rate of cells and cell morphology by scanning and transmission electron microscopy. Results: After US irradiation, the survival rate of tumor cells in the SPFX-added group was 30.9%, significantly lower than 78.7% in the control group (p=0.0002). Most cells in the control group were spherical, but in the SPFX-added group the cells were aspherical. Cell membranes were often ruptured and numerous pores of various sizes were observed. Some cells were totally disintegrated. Conclusion: The most characteristic changes of the synergistic antitumor activities of US and SPFX were on the cell membrane. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -