TY - JOUR T1 - High Frequency of Allele-specific Down-regulation of HLA class I Expression in Lung Cancer Cell Lines JF - Anticancer Research JO - Anticancer Res SP - 1525 LP - 1528 VL - 24 IS - 3A AU - AKIO HIRAKI AU - NOBUHARU FUJII AU - TOMOYUKI MURAKAMI AU - KATSUYUKI KIURA AU - KEISUKE AOE AU - HIROMICHI YAMANE AU - KOZO MASUDA AU - TADASHI MAEDA AU - KAZURO SUGI AU - ZBIGNIEW DARZYNKIEWICZ AU - MITSUNE TANIMOTO AU - MINE HARADA Y1 - 2004/05/01 UR - http://ar.iiarjournals.org/content/24/3A/1525.abstract N2 - Loss or down-regulation of HLA expression has been demonstrated in various types of solid tumors and is considered to be one of the mechanisms of tumor immunoescape. The effectiveness of immunotherapy using tumor-specific antigens (TSA) largely depends on the expression of the appropriate HLA class I alleles on the tumor cells. We analyzed the allele-specific HLA class I surface expression of six lung cancer cell lines using a broad panel of allele-specific monoclonal antibodies, as well as the effect of IFN-γ on HLA expression. Flow cytometric analysis displayed a wide range, from minimal to a high degree of expression of monomorphic HLA class I among the studied cell lines. Allele-specific loss or down-regulation of HLA also was observed in 5 out of 6 cell lines. Our results suggest that lung cancer patients considered for specific immunotherapy should be examined with respect to the expression of specific HLA class I allele binding the TSA. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -