@article {XIE3819, author = {DAN-PING XIE and YI-XI GONG and YING-HUA JIN and CHEN-XI REN and YUE LIU and YING-HAO HAN and MEI-HUA JIN and DAN ZHU and QIU-ZHEN PAN and LI-YUN YU and DONG-SEOK LEE and JAIHYUNG LEE and JIHWAN KIM and YANG HO PARK and JIN WON HYUN and TAEHO KWON and YU-DONG CUI and HU-NAN SUN}, title = {Anti-tumor Properties of Picrasma quassioides Extracts in H-RasG12V Liver Cancer Are Mediated Through ROS-dependent Mitochondrial Dysfunction}, volume = {40}, number = {7}, pages = {3819--3830}, year = {2020}, doi = {10.21873/anticanres.14371}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Picrasma quassioides (PQ) is a traditional Asian herbal medicine with anti-tumor properties that can inhibit the viability of HepG2 liver cancer cells. H-Ras is often mutated in liver cancer, however, the effect of PQ treatment on H-Ras mutated liver cancer is unclear. This study aimed to investigate the role of PQ on ROS accumulation and mitochondrial dysfunction in H-ras mutated HepG2 (HepG2G12V) cells. Materials and Methods: PQ ethanol extract-induced HepG2G12V apoptosis was analyzed by the MTT assay, fluorescence microscopy, flow cytometry and western blotting. Results: PQ treatment affected cell migration and colony formation in HepG2G12V cells. Cleaved-caspase-3, cleaved-caspase-9 and BCL2 associated agonist of cell death (BAD) expression levels were increased, while the levels of B-cell lymphoma-extra large (Bcl-xL) were decreased with PQ treatment. PQ treatment led to a reduction of H-Ras expression levels in liver cancer cells, thus reducing their abnormal proliferation. Furthermore, it led to increased expression levels of Peroxiredoxin VI, which regulates the redox signal in cells. Conclusion: Taken together these results provide a new functional significance for the role of PQ in treating HepG2G12V liver cancer.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/40/7/3819}, eprint = {https://ar.iiarjournals.org/content/40/7/3819.full.pdf}, journal = {Anticancer Research} }