%0 Journal Article %A TSUTOMU NAKAZAWA %A TOSHIHARU MURAKAMI %A ATSUSHI NATSUME %A FUMIHIKO NISHIMURA %A TAKAYUKI MORIMOTO %A RYOSUKE MATSUDA %A MITSUTOSHI NAKAMURA %A SHUICHI YAMADA %A ICHIRO NAKAGAWA %A YOUNG-SOO PARK %A YASUSHI MOTOYAMA %A TAKAHIRO TSUJIMURA %A TOSHIHIKO WAKABAYASHI %A HIROYUKI NAKASE %T KHYG-1 Cells With EGFRvIII-specific CAR Induced a Pseudoprogression-like Feature in Subcutaneous Tumours Derived from Glioblastoma-like Cells %D 2020 %R 10.21873/anticanres.14304 %J Anticancer Research %P 3231-3237 %V 40 %N 6 %X Background/Aim: We previously established a novel type of epidermal growth factor receptor variant III (EGFRvIII)-specific chimeric antigen receptor (CAR)-expressing natural killer (NK) cell line, designated EvCAR-KHYG-1, which inhibited the growth of glioblastoma (GBM) cells in vitro via apoptosis. Materials and Methods: We investigated the cytokine-producing effect of EvCAR-KHYG-1 cells on GBM-like cell lines and their antitumour effect using in vivo xenograft assays. Results: EvCAR-KHYG-1 cells produced interleukin-2, interferon-γ, and tumour necrosis factor-α on EGFRvIII-expressing U87MG cells. In vivo xenograft assays showed that EvCAR-KHYG-1 cells did not reduce the volume of subcutaneous tumours derived from EGFRvIII-expressing U87MG cells but did reduce tumour cell occupancy. Conclusion: EvCAR-KHYG-1 cells led to expression of cellular immunity-related cytokines on EGFRvIII-expressing U87MG in vitro but did not inhibit tumour progression due to the induction of a pseudo progression-like pathological feature. Future studies investigating the effect of different conditions in vivo are required to study the inhibition of tumour progression in GBM. %U https://ar.iiarjournals.org/content/anticanres/40/6/3231.full.pdf