PT  - JOURNAL ARTICLE
AU  - J.-H. ZHANG
AU  - J. WANG
AU  - J. TANG
AU  - B. BARNETT
AU  - J. DICKSON
AU  - N. HAHSIMOTO
AU  - P. WILLIAMS
AU  - W. MA
AU  - W. ZHENG
AU  - T. YONEDA
AU  - S. PAGEAU
AU  - J. CHEN
TI  - Bone Sialoprotein Promotes Bone Metastasis of a Non-bone-seeking Clone of Human Breast Cancer Cells
DP  - 2004 May 01
TA  - Anticancer Research
PG  - 1361--1368
VI  - 24
IP  - 3A
4099  - http://ar.iiarjournals.org/content/24/3A/1361.short
4100  - http://ar.iiarjournals.org/content/24/3A/1361.full
SO  - Anticancer Res2004 May 01; 24
AB  - Bone sialoprotein (BSP) is a major non-collagenous protein in mineralized tissues. BSP is also implied to be involved in tumor metastasis through its unique structure. Using the human breast cancer cell line MDA-231, we established both brain-seeking and bone-seeking cell clones. The brain-seeking cells (MDA-231BR) showed no bone metastasis in an animal model. In this experiment, MDA-231BR cells were transfected with BSP cDNA and inoculated into the hearts of nude mice. All five nude mice which received BSP-transfected MDA-231BR cells developed bone metastases, while no bone lesions were observed in the control group. Histological examination revealed invasion of tumor cells into the endosteal space and erosion of the bone margin. Some animals were crippled due to large lesions. These results suggest that BSP may impart to breast cancer cells the capacity to metastasize and thus play an important role in bone metastasis of malignant tumors. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved