TY - JOUR T1 - Effect of Cycloartanes on Reversal of Multidrug Resistance and Apoptosis Induction on Mouse Lymphoma Cells JF - Anticancer Research JO - Anticancer Res SP - 859 LP - 864 VL - 24 IS - 2B AU - ANA MARGARIDA MADUREIRA AU - GABRIELLA SPENGLER AU - ANNAMARIA MOLNAR AU - ANDREAS VARGA AU - JOSEPH MOLNAR AU - PEDRO M. ABREU AU - MARIA-JOSÉ U. FERREIRA Y1 - 2004/03/01 UR - http://ar.iiarjournals.org/content/24/2B/859.abstract N2 - The ability of fifteen cycloartanes, isolated from Euphorbia species, to reverse multidrug resistance (MDR) and apoptosis induction in L5178Y mouse lymphoma cells, including its multidrug-resistant subline, was studied by flow cytometry. Reversion of MDR was investigated using a standard functional assay with rhodamine 123 as a fluorescent substrate analogue. For the evaluation of apoptosis, the cells were stained with FITC-labeled annexin V and propidium iodide. The majority of the compounds were able to reverse MDR of the tested human MDR1 gene-transfected mouse lymphoma cells. Some of the compounds were able to induce moderate apoptosis in the PAR cell line, but this effect was less effective on multidrug-resistant cells. The results indicate that cycloartanes can be substrates of ABC transporters, which might compete with certain anticancer chemotherapeutics. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -